2026 103 EU PicoQuant

Upgrade of the existing PicoQuant MicroTime 200 to a Luminosa Single Photon Counting Confocal Microscope • Inverted research microscope based on the existing Olympus IX73 body, adapted for compatibility with the Luminosa system • Software-controlled switching between observation modes: confocal, transmission and epifluorescence • FLIMbee Galvo scanner for XY imaging with highly flexible scan speeds, excellent linearity and reproducibility • Z-piezo objective scanner for 3D imaging with 100 µm travel range • Motorized sample stage with software control, travel range 120 mm x 72 mm, compatible with 96-well plates and tiling experiments • Laser Combining Unit (LCU) with pulsed picosecond lasers at 485 nm, 560 nm and 640 nm, suitable for Pulsed Interleaved Excitation (PIE) • Four-color PIE filter set for simultaneous detection at 405 + 485 + 560 + 640 nm excitation with detection bandpasses 445/30, 520/35, 595/40 and 690/70 nm • Main Optical Unit (MOU) with complete detection beam path, electronics, interlock, auto-alignment beam path and liquid cooling, hosting up to 4 detectors • TCSPC electronics with 5 ps time resolution, 65536 channels and 32-bit depth, suitable for TTTR mode and custom programming • 2x Excelitas Single Photon Avalanche Diode (SPAD) detectors • Polarizing beam splitter cube for anisotropy measurements • Variable PSF feature for fine-tuning of the observation volume size for FCS and smFRET experiments • LED-based epifluorescence illumination with software control and filter cubes for DAPI, eGFP and mCherry/TexasRed • DIC illumination mode with motorized DIC prism, enabling software-controlled pixel-precise FLIM-DIC overlays • Luminosa software with full Python interface for automated and feedback-based experiment control • 4 days on-site installation and training by PicoQuant engineers